H020 Hansatech Gas-Phase Oxygen Electrode Systems Leafview 1 System

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Leafview 1 is an entry-level system for studies of photosynthesis and respiration from gas-phase, solid-state samples under actinic illumination. The system is ideally suited to teaching environments for under and post-grad plant biology studies of the photosynthetic processes but is equally at home in research facilities where demands on equipment performance are high.

Samples typically consist of leafdiscs which are either cut from a broad leaf or made up of a “mat” of smaller leaves to form a circular disc of 10cm2. Changes in the oxygen content of the sealed sample chamber are determined by the integral oxygen electrode mounted in the base of the chamber.

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Code: H020

The individual components of the Leafview 1 System are described below:

S1 Oxygen Electrode Disc
Since its original design in the early 1970s by Tom Delieu and David Walker, the S1 Clark-type oxygen electrode disc remains largely unchanged – a true testament to the quality and reliability of the sensor. The S1 consists of a platinum cathode and silver anode set into an epoxy resin disc and is prepared for use by trapping a layer of 50% saturated KCl solution beneath an oxygen-permeable PTFE membrane. A paper spacer placed beneath the membrane acts as a wick to provide a uniform layer of electrolyte between anode and cathode.

When a small voltage is applied across these electrodes (with the platinum negative with respect to the silver), the current which flows is at first negligible and the platinum becomes polarised (i.e. it adopts the externally applied potential). As this potential is increased to 700 mV, oxygen is reduced at the platinum surface, initially to hydrogen peroxide H2O2 so that the polarity tends to discharge as electrons are donated to oxygen (which acts as an electron acceptor). The current which then flows is stoichiometrically related to the oxygen consumed at the cathode.

When connected to the electrode control unit, the S1 provides a fast, effective and accurate method of detecting small changes in oxygen concentration.

 

 

 

 

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