H016 Hansatech Oxytherm+P System

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The Oxytherm+ P advanced oxygen electrode system provides PC control of oxygen uptake or evolution measurements across a broad range of research applications up to 100% oxygen concentration. Oxytherm+ P is the ideal choice for measurements of photosynthetic oxygen evolution in isolated chloroplasts or algae/cyanobacteria suspensions where an actinic light source is necessary to drive photosynthetic reactions in the sample.

The Oxytherm+ P system consists of a highly sensitive S1 Clark-type polarographic oxygen electrode disc mounted within an electronic electrode chamber and connected to the Oxytherm+ P electrode control unit. The electrode chamber provides a highly versatile solution to measurements of dissolved oxygen in liquid-phase samples of between 0.2 – 2.5ml with integral precise solid-state temperature control configured from within the supplied software.

Oxytherm+P is fitted with 2 software-controlled, high-intensity white LED light sources mounted against the outer wall of the reaction vessel which may be used to perform light response measurements for photosynthetic samples.

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Category:

Code: H016

The individual components of the Oxytherm+ P System are described below:

S1 Oxygen Electrode Disc
Since its original design in the early 1970s by Tom Delieu and David Walker, the S1 Clark-type oxygen electrode disc remains largely unchanged – a true testament to the quality and reliability of the sensor. The S1 consists of a platinum cathode and silver anode set into an epoxy resin disc and is prepared for use by trapping a layer of 50% saturated KCl solution beneath an oxygen-permeable PTFE membrane. A paper spacer placed beneath the membrane acts as a wick to provide a uniform layer of electrolyte between anode and cathode.

When a small voltage is applied across these electrodes (with the platinum negative with respect to the silver), the current which flows is at first negligible and the platinum becomes polarised (i.e. it adopts the externally applied potential). As this potential is increased to 700 mV, oxygen is reduced at the platinum surface, initially to hydrogen peroxide H2O2 so that the polarity tends to discharge as electrons are donated to oxygen (which acts as an electron acceptor). The current which then flows is stoichiometrically related to the oxygen consumed at the cathode.

When connected to the electrode control unit, the S1 provides a fast, effective and accurate method of detecting small changes in oxygen concentration.

 

 

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